The Principle and Application of Rapid Detection Cassette for Pathogenic Bacteria

The rapid detection cassette for pathogenic bacteria usually adopts immunochromatographic technology, which uses antigen antibody reaction for qualitative detection. When a certain pathogenic bacteria is present in the test sample, the antigen in the sample will bind with the specific antibody in the reagent, forming an antigen antibody complex. The complex will undergo color changes under specific conditions to determine the presence of this pathogenic bacterium in the sample. Taking Listeria monocytogenes as an example.

Listeria monocytogenes, abbreviated as Listeria mono., is a Gram positive small bacterium that is a zoonotic pathogen. It can cause listeriosis in humans and animals. This bacterium is an intracellular parasite, and its clearance by the host mainly relies on cellular immune function. Therefore, listeriosis mainly occurs in newborns, the elderly, and those with low immune function. Listeria monocytogenes is mainly infected through the fecal oral route, and can also enter the body through the eyes, damaged skin, and mucous membranes, causing infection.

Application:
The rapid detection cassette can be used for rapid screening and detection of Listeria monocytogenes in food, environment, and clinical samples, providing scientific research and disease control.

Product features:
Fast: The detection process is simple and can usually be completed in a short period of time, improving detection efficiency
Accurate: High specificity antibodies are used to ensure the accuracy of the test results.
Easy to use: The reagent is stable, easy to operate, and does not require complex pre-processing steps.
Sensitive: capable of detecting low concentrations of Listeria monocytogenes, improving the sensitivity of detection.

Operation method:
1) Sample preparation: Collect samples according to relevant standards or regulations, and ensure the representativeness and completeness of the samples.
2) Reagent preparation: Return the reagent to room temperature and shake well to ensure its uniformity.
3) Sample processing: Select appropriate processing methods based on the sample type, such as dilution, filtration, etc., to ensure sufficient reaction between the sample and the reagent.
4) Sample addition: Drop the processed sample onto the designated area on the cassette.
5) Result interpretation: Observe the color change on the reagent cassette within the specified time and judge the result according to the interpretation criteria in the instruction manual.

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