Overview:
Aflatoxin is a toxic metabolite of a class of fungi such as Aspergillus flavus and Aspergillus parasiticus, which has strong carcinogenicity and is mainly present in grains, peanuts, nuts, cottonseeds, feed, vegetable oils, animal tissues, and blood. Among them, aflatoxin B1 ranks first in terms of toxicity, carcinogenicity, and pollution frequency.
Application: The immunoaffinity column can selectively adsorb aflatoxins (B1, B2, G1, G2) in the sample solution, thereby providing a highly targeted purification effect on the sample. The purified sample solution can be directly used for HPLC analysis. The combination of affinity column and HPLC can achieve rapid determination, improve signal-to-noise ratio, and enhance the accuracy of the detection method.
Principle:
The basis of determination is antigen antibody reaction. The antibody is connected to the column, and the aflatoxin in the sample is extracted, filtered, diluted, and slowly passed through the aflatoxin immunoaffinity column. The endotoxin binds to the antibody in the immunoaffinity column, and then the immunoaffinity column is washed to remove other unrelated substances that have not been bound. Wash aflatoxin with methanol and inject it into the analytical instrument for detection.
Features:
1 High specificity, capable of enriching and purifying trace toxins.
- Penetrating plunger, easy to use, can pass through the column in 5-10 minutes.
- High accuracy, the addition recovery rate can reach 90% -110%.
- Widely used, it is the mainstream purification tool for official testing methods in various countries.
- Complies with the GB5009 series of toxin detection standards.
- Good stability, with a coefficient of variation of no more than 10% for repeated testing.